Vitamin E protects against oxidative damage caused by cadmium in the blood of rats
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Mehmet Kanter 1 * , Burhan Aksu 2, Meryem Akpolat 1, Yeter Topcu Tarladacalisir 1, Cevat Aktas 1, Hamdi Uysal 3
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1 Department of Histology & Embryology, Faculty of Medicine, Trakya University, Edirne, Turkey2 Department of Pediatric Surgery, Faculty of Medicine, Trakya University, Edirne, Turkey3 Department of Biochemistry, Faculty of Veterinary, Ankara University, Ankara* Corresponding Author

Abstract

Aim: The protective effect of vitamin E (vit E) on cadmium (Cd) induced oxidative stress was studied in the blood of rats. Methods: The rats were randomly divided in to three experimental groups: Control, Cd treated and Cd + vit E treated, each containing 10 animals. The Cd treated and Cd + vit E treated groups were injected subcutaneously daily with CdCl2 dissolved in isotonic NaCl in the amount of 2 mL/kg for 20 days, resulting in a dosage of 0.49 mg Cd/kg/d. In addition, Cd + vit E treated group received intramuscular injection of 150 mg/kg vit E until the end of the study. Results: Cd treatment increased significantly malondialdehyde (MDA) levels and the antioxidant enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in plasma and erythrocytes compared to the control group. Cd + vit E treatment, decreased significantly elevated MDA levels in plasma and erythrocyte and also reduced significantly the enhanced antioxidant levels. Cd treatment increased significantly the activity of iron levels in the plasma compared to the control group. Cd + vit E treatment, decreased the activity of iron levels in the plasma compared to the Cd treated group. In the control group, the histology of erythrocytes was normal. In Cd treated group, there was marked membrane destruction and there were hemolytic changes in erythrocytes. In Cd + vit E treated group, these changes were less than Cd treated group. Conclussion: Our results show that vit E exerts a protective effect against cadmium toxicity.

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Original Article

EUR J GEN MED, 2009, Volume 6, Issue 3, 154-160

https://doi.org/10.29333/ejgm/82661

Publication date: 15 Jul 2009

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