Increased Sister Chromatid Exchanges in Patients with Gastrointestinal Cancers and in their First-Degree Relatives
Taner Turgut 1, Mehmet Yaşar 2, Kürşat Oğuz Yaykaşlı 3 * , Ertuğrul Ertaş 4, Fatma Sılan 5
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1 Department of General Surgery, Derince Training and Research Hospital, Kocaeli, Turkey2 Department of General Surgery, Düzce University Medical Faculty, Turkey3 Department of Medical Genetics, Düzce University Medical Faculty, Turkey4 Department of General Surgery, Ankara Training and Research Hospital, Ankara, Turkey5 Department of Medical Genetics, Çanakkale Onsekiz Mart University Medical Faculty, Çanakkale, Turkey* Corresponding Author

Abstract

Gastrointestinal Cancers (GICs) are the most important causes of mortality and morbidity in industrialized world. Sister chromatid exchange (SCE), as an index of chromosomal instability, involves cancer. The aim of this study is to determine whether SCE frequency is a heritable factor for GIC or not. The study groups consisted of 15 gastrointestinal carcinoma patients, 13 patient relatives and 15 healthy subjects as the control group. After collection of 2 ml peripheral blood, lymphocytes were cultured for 3 days and sister chromatid exchange (SCE), mitotic index, and replication index were analyzed. SCE was significantly increased (p<0.01) in patients (16.06±22.37) and in their relatives (5.23±2.64) compared with controls (3.51±1.58). There was no significant difference between patients’ relatives and control group in terms of the incidence of SCE frequency. Mitotic index was significantly decreased (p<0.05, p<0.01) in patients (5.4±3.13) compared with healthy relatives (7.15±2.15) and controls (9.00±2.26). Replication index was also significantly lower (p<0.01) in patients (1.39±0.35) and in their relatives (1.7±0.21) compared with controls (2.04±1.13). The results of this study indicate that SCE is a heritable factor for GICs. Increased SCE reflects genomic instability, which is an important factor in carcinogenesis. Although the most putative factors causing genomic instability are epigenetics marks, further studies in combination with epigenetic modifications are needed using more subjects.

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Original Article

EUR J GEN MED, 2014, Volume 11, Issue 2, 94-98

https://doi.org/10.15197/sabad.1.11.46

Publication date: 15 Apr 2014

Article Views: 1206

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