Examination of Apoptotic Effects of High-Dose Toluene on the Brain Cortex and Cerebellum Tissue During the Acute Phase: an experimental study
Ufuk Tas 1 * , Murat Ayan 1, Tuncay Kuloglu 2, Mustafa Suren 1, Duygu Cakıl 1, Birsen Ozyurt 1, Dürdane Aksoy Bekar 1, Mustafa Sarsılmaz 3
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1 Gazıosmanpaşa University, Faculty of Medicine,Tokat, Turkey
2 Firat University, Faculty of Medicine, Elazig, Turkey
3 Sifa University, Faculty of Medicine, Izmir, Turkey
* Corresponding Author


The aim of this study is to examine the apoptotic effects of high-dose toluene on rat brain cortex and cerebellum tissues during the acute phase. Fourteen albino Wistar rats (180-200 g) were divided into two equal groups (n : 7). While Group I was used as a control group, Group II was applied to a high dose of toluene (5200 mg / kg / gavage). At the end of a three-hour experimental period brain tissue samples were taken from the killed animals and tissues were fixed in % 10 neutral formalin, then, embedded in paraffin and sectioned (thickness, 5 μm) to determine the immune reactivity of Bax, sections were stained immunohistochemically with avidin biotin-peroxidase method. At the same time the TUNEL method was used for detection of apoptosis in the brain cortex and cerebellum. As a result of the study, increased Bax immune reactivity was seen in the brain cortex (+++) and cerebellum (++++) of toluene treated rats compared to control. The numbers of TUNEL-positive cells were significantly higher in the cerebellum tissues of animals exposed to toluene than the control. Nevertheless there was not a significantly difference in terms of TUNEL positivity between the brain cortex of control and toluene exposed animals. As a result of this study it was shown that a high-dose of toluene can trigger apoptosis of the brain cortex and cerebellum in a very short period of time.


This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Original Article


Eur J Gen Med, 2012 - Volume 9 Issue 4, pp. 235-240

Publication date: 10 Oct 2012

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