Examination of Apoptotic Effects of High-Dose Toluene on the Brain Cortex and Cerebellum Tissue During the Acute Phase: an experimental study
Ufuk Tas 1 * , Murat Ayan 1, Tuncay Kuloglu 2, Mustafa Suren 1, Duygu Cakıl 1, Birsen Ozyurt 1, Dürdane Aksoy Bekar 1, Mustafa Sarsılmaz 3
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1 Gazıosmanpaşa University, Faculty of Medicine,Tokat, Turkey
2 Firat University, Faculty of Medicine, Elazig, Turkey
3 Sifa University, Faculty of Medicine, Izmir, Turkey
* Corresponding Author


The aim of this study is to examine the apoptotic effects of high-dose toluene on rat brain cortex and cerebellum tissues during the acute phase. Fourteen albino Wistar rats (180-200 g) were divided into two equal groups (n : 7). While Group I was used as a control group, Group II was applied to a high dose of toluene (5200 mg / kg / gavage). At the end of a three-hour experimental period brain tissue samples were taken from the killed animals and tissues were fixed in % 10 neutral formalin, then, embedded in paraffin and sectioned (thickness, 5 μm) to determine the immune reactivity of Bax, sections were stained immunohistochemically with avidin biotin-peroxidase method. At the same time the TUNEL method was used for detection of apoptosis in the brain cortex and cerebellum. As a result of the study, increased Bax immune reactivity was seen in the brain cortex (+++) and cerebellum (++++) of toluene treated rats compared to control. The numbers of TUNEL-positive cells were significantly higher in the cerebellum tissues of animals exposed to toluene than the control. Nevertheless there was not a significantly difference in terms of TUNEL positivity between the brain cortex of control and toluene exposed animals. As a result of this study it was shown that a high-dose of toluene can trigger apoptosis of the brain cortex and cerebellum in a very short period of time.


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Article Type: Original Article


EUR J GEN MED, 2012 - Volume 9 Issue 4, pp. 235-240

Publication date: 10 Oct 2012

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