In late 2019 on 11th March 2020 a novel coronavirus, SARS-CoV-2 causing Coronavirus disease 2019 (COVID-19) appeared in Wuhan, China and World Health Organisation declared it to have developed pandemic status. Although there are many detection techniques for the disease to control the pandemic such as RT-PCR, serological methods, or fast antigen tests, the actual problem is the prevention of the disease. The nasal cavity and rhino pharynx are key sites of the initial replication of SARS-CoV-2. In this article, the effectiveness of destrovir spray was investigated by creating a mechanical barrier in the nasal and oral mucosa, which are the entry points of the virus into the body, and to destroy the virus that comes into contact with this barrier. In the presented study, quantitation cycle (Cq) and irradiation values (RFU) of 12 different high-positive patients (Cq≤25) after application of both RT-PCR and destrovir spray were determined at different concentrations (10%, 20%, 30%, 40%, and 50%) aimed to evaluate its effectiveness. According to the results obtained by comparing the effectiveness of disinfectant with 70% ethyl alcohol on the 46 patient samples with Cq<20, 46 patient samples with Cq values between 20-25, and 46 patient samples with Cq≥25 including different variants are examined. A total of 138 COVID-19 patient samples were treated with 5% and 10% ratios of destrovir spray. Its effectiveness on Cq values and RFU was evaluated and it was aimed to compare the effectiveness of this evaluation with 96% ethyl alcohol used in the same samples.