Internal jugular vein cannulation has become the preferred approach for temporary vascular access for hemodialysis. Internal jugular vein cannulation is associated with a high rate of successful catheter placement. However, significant complications such as internal carotid artery (ICA) puncture, vessel erosion, thrombosis and infection can occur. We present one case of Horner’s syndrome (without arterial punction) occurring following internal jugular venous cannulation. We suggested that, clinicians need to be aware of the risk of Horner’s syndrome as a possible complication of percutaneous hemodialysis catheterization via the internal jugular vein and should avoid repeated manipulations.