Abstract
The novel coronavirus known to have brought the world to a standstill is responsible for many deaths throughout the globe as of now. The causative agent (SARS-CoV-2) for coronavirus disease 2019 (COVID-19) has been recognized as a zoonotic transfer. Although, the medium of animal-human transmission is still unknown, bats maybe a potential reservoir of this novel strain. Due to its high rate of transmission the most favorable way of limiting the outbreak’s extent is by early diagnosis followed by isolation of the infected individuals. So far, the most widely used diagnosis methods are RT-qPCR which detects specific sequences of the viral RNA. Some other methods include serological tests and the recently introduced CRISPR-CAS-12 based assays.
As of now, no specific therapeutic treatments are known for COVID-19 however the use of some broad-spectrum antiviral drugs and convalescent plasma therapy have demonstrated positive outcomes. Apart from these treatments, vaccine development for SARS-CoV-2 is also in progress by 17 known companies. This article provides a comprehensive insight on the recently emerged Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) including its origin, transmission mechanism, pathophysiology and updated treatment methods.
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Article Type: Review Article
ELECTRON J GEN MED, Volume 17, Issue 6, December 2020, Article No: em251
https://doi.org/10.29333/ejgm/8258
Publication date: 11 May 2020
Article Views: 7329
Article Downloads: 3789
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References
- Zhu N, Zhang D, Wang W, Li X, Yang B, Song J, et al. A novel coronavirus from patients with pneumonia in China, 2019. New England Journal of Medicine. 2020 Jan 24. https://doi.org/10.1056/NEJMoa2001017 PMid:31978945 PMCid:PMC7092803
- Cui J, Li F, Shi ZL. Origin and evolution of pathogenic coronaviruses. Nature reviews Microbiology. 2019 Mar;17(3):181-92. https://doi.org/10.1038/s41579-018-0118-9 PMid:30531947 PMCid:PMC7097006
- Woo PC, Lau SK, Lam CS, Lau CC, Tsang AK, Lau JH, et al. Discovery of seven novel Mammalian and avian coronaviruses in the genus deltacoronavirus supports bat coronaviruses as the gene source of alphacoronavirus and betacoronavirus and avian coronaviruses as the gene source of gammacoronavirus and deltacoronavirus. Journal of virology. 2012 Apr 1;86(7):3995-4008. https://doi.org/10.1128/JVI.06540-11 PMid:22278237 PMCid:PMC3302495
- Wang LF, Shi Z, Zhang S, Field H, Daszak P, Eaton BT. Review of bats and SARS. Emerging infectious diseases. 2006 Dec;12(12):1834. https://doi.org/10.3201/eid1212.060401 PMid:17326933 PMCid:PMC3291347
- Chen Y, Guo D. Molecular mechanisms of coronavirus RNA capping and methylation. VirologicaSinica. 2016 Feb 1;31(1):3-11. https://doi.org/10.1007/s12250-016-3726-4 PMid:26847650 PMCid:PMC7091378
- Snijder EJ, Van Der Meer Y, Zevenhoven-Dobbe J, Onderwater JJ, van der Meulen J, Koerten HK, Mommaas AM. Ultrastructure and origin of membrane vesicles associated with the severe acute respiratory syndrome coronavirus replication complex. Journal of virology. 2006 Jun 15;80(12):5927-40. https://doi.org/10.1128/JVI.02501-05 PMid:16731931 PMCid:PMC1472606
- Chen Y, Liu Q, Guo D. Emerging coronaviruses: genome structure, replication, and pathogenesis. Journal of medical virology. 2020 Apr;92(4):418-23. https://doi.org/10.1002/jmv.25681 PMid:31967327 PMCid:PMC7167049
- Tyrrell DA, Bynoe ML. Cultivation of a novel type of common-cold virus in organ cultures. British medical journal. 1965 Jun 5;1(5448):1467. https://doi.org/10.1136/bmj.1.5448.1467 PMid:14288084 PMCid:PMC2166670
- Perlman P. Fields Virology. 2nd ed. 2013:825-58.
- Su S, Wong G, Shi W, Liu J, Lai AC, Zhou J, et al. Epidemiology, genetic recombination, and pathogenesis of coronaviruses. Trends in microbiology. 2016 Jun 1;24(6):490-502. https://doi.org/10.1016/j.tim.2016.03.003 PMid:27012512 PMCid:PMC7125511
- Forni D, Cagliani R, Clerici M, Sironi M. Molecular evolution of human coronavirus genomes. Trends in microbiology. 2017 Jan 1;25(1):35-48. https://doi.org/10.1016/j.tim.2016.09.001 PMid:27743750 PMCid:PMC7111218
- Fehr AR, Perlman S. Coronaviruses: an overview of their replication and pathogenesis. In Coronaviruses 2015 (pp. 1-23). Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2438-7_1 PMid:25720466 PMCid:PMC4369385
- World Health Organization. Severe Acute Respiratory Syndrome (SARS)–multi-country outbreak–update 36. Outbreak reported March. 2003 Apr 8;19.
- Parry J. WHO investigates China’s fall in SARS cases. BMJ: British Medical Journal. 2003 Jun 14;326(7402):1285. https://doi.org/10.1136/bmj.326.7402.1285-c PMid:12805143 PMCid:PMC1126174
- Guan Y, Zheng BJ, He YQ, Liu XL, Zhuang ZX, Cheung CL, et al. Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China. Science. 2003 Oct 10;302(5643):276-8. https://doi.org/10.1126/science.1087139 PMid:12958366
- Perlman S, Netland J. Coronaviruses post-SARS: update on replication and pathogenesis. Nature reviews microbiology. 2009 Jun;7(6):439-50. https://doi.org/10.1038/nrmicro2147 PMid:19430490 PMCid:PMC2830095
- Lau SK, Li KS, Huang Y, Shek CT, Tse H, Wang M, et al. Ecoepidemiology and complete genome comparison of different strains of severe acute respiratory syndrome-related Rhinolophus bat coronavirus in China reveal bats as a reservoir for acute, self-limiting infection that allows recombination events. Journal of virology. 2010 Mar 15;84(6):2808-19. https://doi.org/10.1128/JVI.02219-09 PMid:20071579 PMCid:PMC2826035
- Chan-Yeung M, Xu RH. SARS: Epidemiology. Respirology 8: S9–S14. https://doi.org/10.1046/j.1440-1843.2003.00518.x PMid:15018127 PMCid:PMC7169193
- Zaki AM, Van Boheemen S, Bestebroer TM, Osterhaus AD, Fouchier RA. Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. New England Journal of Medicine. 2012 Nov 8;367(19):1814-20. https://doi.org/10.1056/NEJMoa1211721 PMid:23075143
- Lee J, Chowell G, Jung E. A dynamic compartmental model for the Middle East respiratory syndrome outbreak in the Republic of Korea: a retrospective analysis on control interventions and superspreading events. Journal of theoretical biology. 2016 Nov 7;408:118-26. https://doi.org/10.1016/j.jtbi.2016.08.009 PMid:27521523 PMCid:PMC7094115
- Lee JY, Kim YJ, Chung EH, Kim DW, Jeong I, Kim Y, et al. The clinical and virological features of the first imported case causing MERS-CoV outbreak in South Korea, 2015. BMC infectious diseases. 2017 Dec 1;17(1):498. https://doi.org/10.1186/s12879-017-2576-5 PMid:28709419 PMCid:PMC5512736
- Huang C, Wang Y, Li X, Ren L, Zhao J, Hu Y, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. The Lancet. 2020 Feb 15;395(10223):497-506. https://doi.org/10.1016/S0140-6736(20)30183-5
- Chan JF, Yuan S, Kok KH, To KK, Chu H, Yang J, et al. A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. The Lancet. 2020 Feb 15;395(10223):514-23. https://doi.org/10.1016/S0140-6736(20)30154-9
- Yoo JH. The fight against the 2019-nCoV outbreak: an arduous march has just begun. Journal of Korean Medical Science. 2019 Dec 24;35(4). https://doi.org/10.3346/jkms.2020.35.e56 PMid:31997618 PMCid:PMC6995816
- Beniac DR, Andonov A, Grudeski E, Booth TF. Architecture of the SARS coronavirus prefusion spike. Nature structural & molecular biology. 2006 Aug;13(8):751-2. https://doi.org/10.1038/nsmb1123 PMid:16845391 PMCid:PMC7097490
- Delmas BE, Laude HU. Assembly of coronavirus spike protein into trimers and its role in epitope expression. Journal of Virology. 1990 Nov 1;64(11):5367-75. https://doi.org/10.1128/JVI.64.11.5367-5375.1990 PMid:2170676 PMCid:PMC248586
- Nal B, Chan C, Kien F, Siu L, Tse J, Chu K, et al. Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E. Journal of general virology. 2005 May 1;86(5):1423-34. https://doi.org/10.1099/vir.0.80671-0 PMid:15831954
- Neuman BW, Kiss G, Kunding AH, Bhella D, Baksh MF, Connelly S, et al. A structural analysis of M protein in coronavirus assembly and morphology. Journal of structural biology. 2011 Apr 1;174(1):11-22. https://doi.org/10.1016/j.jsb.2010.11.021 PMid:21130884 PMCid:PMC4486061
- DeDiego ML, Álvarez E, Almazán F, Rejas MT, Lamirande E, Roberts A, et al. A severe acute respiratory syndrome coronavirus that lacks the E gene is attenuated in vitro and in vivo. Journal of virology. 2007 Feb 15;81(4):1701-13. https://doi.org/10.1128/JVI.01467-06 PMid:17108030 PMCid:PMC1797558
- Nieto-Torres JL, DeDiego ML, Verdia-Baguena C, Jimenez-Guardeno JM, Regla-Nava JA, Fernandez-Delgado R, et al. Severe acute respiratory syndrome coronavirus envelope protein ion channel activity promotes virus fitness and pathogenesis. PLoS pathogens. 2014 May;10(5). https://doi.org/10.1371/journal.ppat.1004077 PMid:24788150 PMCid:PMC4006877
- Bogoch II, Watts A, Thomas-Bachli A, Huber C, Kraemer MU, Khan K. Pneumonia of Unknown Etiology in Wuhan, China: Potential for International Spread Via Commercial Air Travel. Journal of Travel Medicine. 2020 Jan 14. https://doi.org/10.1093/jtm/taaa008 PMid:31943059 PMCid:PMC7107534
- Lu R, Zhao X, Li J, Niu P, Yang B, Wu H, et al. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. The Lancet. 2020 Feb 22;395(10224):565-74. https://doi.org/10.1016/S0140-6736(20)30251-8
- Wu Y, Ho W, Huang Y, Jin DY, Li S, Liu SL, et al. SARS-CoV-2 is an appropriate name for the new coronavirus. The Lancet. 2020 Mar 21;395(10228):949-50. https://doi.org/10.1016/S0140-6736(20)30557-2
- Zhang YZ, Holmes EC. A genomic perspective on the origin and emergence of sars-cov-2. Cell. 2020 Mar 26. https://doi.org/10.1016/j.cell.2020.03.035 PMid:32220310 PMCid:PMC7194821
- Wan Y, Shang J, Graham R, Baric RS, Li F. Receptor recognition by the novel coronavirus from Wuhan: an analysis based on decade-long structural studies of SARS coronavirus. Journal of virology. 2020 Mar 17;94(7). https://doi.org/10.1128/JVI.00127-20
- Zhang Z, Wu Q, Zhang T. Pangolin homology associated with 2019-nCoV. bioRxiv. 2020 Jan 1. https://doi.org/10.1101/2020.02.19.950253
- Coronavirus [Internet]. Who.int. 2020 [cited 17 April 2020]. Available at: https://www.who.int/emergencies/diseases/novel-coronavirus-2019
- Shereen M, Khan S, Kazmi A, Bashir N, Siddique R. COVID-19 infection: Origin, transmission, and characteristics of human coronaviruses. Journal of Advanced Research. 2020;24:92. https://doi.org/10.1016/j.jare.2020.03.005 PMid:32257431 PMCid:PMC7113610
- Wang C, Horby P, Hayden F, Gao G. A novel coronavirus outbreak of global health concern. The Lancet. 2020;395(10223):470, 471. https://doi.org/10.1016/S0140-6736(20)30185-9
- Zhou P, Yang X, Wang X, Hu B, Zhang L, Zhang W, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020;579(7798):270. https://doi.org/10.1038/s41586-020-2012-7 PMid:32015507 PMCid:PMC7095418
- Wu F, Zhao S, Yu B, Chen Y, Wang W, Song Z, et al. A new coronavirus associated with human respiratory disease in China. Nature. 2020;579(7798):265. https://doi.org/10.1038/s41586-020-2008-3 PMid:32015508 PMCid:PMC7094943
- Kutter J, Spronken M, Fraaij P, Fouchier R, Herfst S. Transmission routes of respiratory viruses among humans. Current Opinion in Virology. 2018;28:142. https://doi.org/10.1016/j.coviro.2018.01.001 PMid:29452994 PMCid:PMC7102683
- Little L. Coronavirus symptoms day-by-day - when to expect signs of killer bug to strike [Internet]. The US Sun. 2020 [cited 12 April 2020]. Available at: https://www.the-sun.com/news/554654/coronavirus-symptoms-day-by-day-when-to-expect-signs-of-killer-bug-to-strike/
- [Internet]. Cdc.gov. 2020 [cited 12 April 2020]. Available at: https://www.cdc.gov/coronavirus/2019-ncov/downloads/2019-ncov-factsheet.pdf
- Pregnant women with coronavirus infection can pass it to their babies, study finds [Internet]. Los Angeles Times. 2020 [cited 18 April 2020]. Available at: https://www.latimes.com/science/story/2020-03-26/coronavirus-pregnant-women-with-covid-19-babies
- Karimi-Zarchi M, Neamatzadeh H, Dastgheib S, Abbasi H, Mirjalili S, Behforouz A, et al. Vertical Transmission of Coronavirus Disease 19 (COVID-19) from Infected Pregnant Mothers to Neonates: A Review. Fetal and Pediatric Pathology. 2020:1. https://doi.org/10.1080/15513815.2020.1747120 PMCid:PMC7157948
- How a Bronx Zoo tiger was tested for coronavirus [Internet]. New York Post. 2020 [cited 18 April 2020]. Available at: https://nypost.com/2020/04/12/how-a-bronx-zoo-tiger-was-tested-for-coronavirus/
- The Real Reason Veterinarians Gave a Tiger a Covid-19 Test ... [Internet]. [cited 2020Apr27]. Available at: https://www.wired.com/story/tiger-coronavirus-bronx-zoo/
- Coronavirus Update (Live): 1,830,982 Cases and 113,008 Deaths from COVID-19 Virus Pandemic - Worldometer [Internet]. Worldometers.info. 2020 [cited 12 April 2020]. Available at: https://www.worldometers.info/coronavirus/
- Peiris JS, Yuen KY, Osterhaus AD, Stöhr K. The severe acute respiratory syndrome. New England Journal of Medicine. 2003 Dec 18;349(25):2431-41. https://doi.org/10.1056/NEJMra032498 PMid:14681510
- Tai W, He L, Zhang X, Pu J, Voronin D, Jiang S, et al. Characterization of the receptor-binding domain (RBD) of 2019 novel coronavirus: implication for development of RBD protein as a viral attachment inhibitor and vaccine. Cellular & Molecular Immunology. 2020. https://doi.org/10.1038/s41423-020-0400-4 PMCid:PMC7091888
- Holshue M, DeBolt C, Lindquist S, Lofy K, Wiesman J, Bruce H, et al. First Case of 2019 Novel Coronavirus in the United States. New England Journal of Medicine. 2020;382(10). https://doi.org/10.1056/NEJMoa2001191 PMid:32004427 PMCid:PMC7092802
- With Europe’s first confirmed coronavirus cases, how is France dealing with the disease? [Internet]. France 24. 2020 [cited 12 April 2020]. Available at: https://www.france24.com/en/20200126-france-china-coronavirus-china-wuhan-disease-epidemic-buzyn-who-paris-hidalgo-lunar-new-year-bordeaux
- First confirmed case of novel coronavirus in Australia [Internet]. Australian Government Department of Health. 2020 [cited 12 April 2020]. Available at: https://www.health.gov.au/ministers/the-hon-greg-hunt-mp/media/first-confirmed-case-of-novel-coronavirus-in-australia
- Contributor S. Scientists figure out how new coronavirus breaks into human cells [Internet]. livescience.com. 2020 [cited 13 April 2020]. Available at: https://www.livescience.com/how-coronavirus-infects-cells.html
- Wrapp D, Wang N, Corbett K, Goldsmith J, Hsieh C, Abiona O, et al. Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science. 2020;367(6483):1260. https://doi.org/10.1126/science.abb2507 PMid:32075877 PMCid:PMC7164637
- Yan R, Zhang Y, Li Y, Xia L, Guo Y, Zhou Q. Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2. Science. 2020;367(6485):1444. https://doi.org/10.1126/science.abb2762 PMid:32132184 PMCid:PMC7164635
- Why does the coronavirus spread so easily between people? [Internet]. Nature.com. 2020 [cited 13 April 2020]. Available at: https://www.nature.com/articles/d41586-020-00660-x
- Coutard B, Valle C, de Lamballerie X, Canard B, Seidah N, Decroly E. The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade. Antiviral Research. 2020;176:104742. https://doi.org/10.1016/j.antiviral.2020.104742 PMid:32057769 PMCid:PMC7114094
- Belouzard S, Millet J, Licitra B, Whittaker G. Mechanisms of Coronavirus Cell Entry Mediated by the Viral Spike Protein. Viruses. 2012;4(6):1014. https://doi.org/10.3390/v4061011 PMid:22816037 PMCid:PMC3397359
- (COVID-19) C, Health E, Disease H, Disease L, Management P, Conditions S et al. What Does Coronavirus Do to Your Body? [Internet]. WebMD. 2020 [cited 13 April 2020]. Available at: https://www.webmd.com/lung/coronavirus-covid-19-affects-body#1
- M.N. Iqbal H, Romero-Castillo K, Bilal M, Parra-Saldivar R. The Emergence of Novel-Coronavirus and its Replication Cycle - An Overview. Journal of Pure and Applied Microbiology. 2020;14(1):16. https://doi.org/10.22207/JPAM.14.1.03
- Zhou F, Yu T, Du R, Fan G, Liu Y, Liu Z, et al. Clinical course and risk factors for mortality of adult inpatients with COVID-19 in Wuhan, China: a retrospective cohort study. The Lancet. 2020;395(10229):1054-62. https://doi.org/10.1016/S0140-6736(20)30566-3
- Coronavirus Incubation Period (COVID-19) - Worldometer [Internet]. Worldometers.info. 2020 [cited 18 April 2020]. Available at: https://www.worldometers.info/coronavirus/coronavirus-incubation-period/
- Coronavirus Disease 2019 (COVID-19) [Internet]. Centers for Disease Control and Prevention. 2020 [cited 18 April 2020]. Available at: https://www.cdc.gov/coronavirus/2019-ncov/hcp/clinical-guidance-management-patients.html
- Guan W, Ni Z, Hu Y, Liang W, Ou C, et al. Clinical Characteristics of Coronavirus Disease 2019 in China. New England Journal of Medicine. 2020. Available at: https://www.nejm.org/doi/full/10.1056/NEJMoa2002032
- Chen N, Zhou M, Dong X, Qu J, Gong F, et al. Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study. The Lancet, 2020;395(10223):507. https://doi.org/10.1016/S0140-6736(20)30211-7
- Wang D, Hu B, Hu C, Zhu F, Liu X, et al. Clinical Characteristics of 138 Hospitalized Patients With 2019 Novel Coronavirus–Infected Pneumonia in Wuhan, China. JAMA, 2020;323(11):1061. https://doi.org/10.1001/jama.2020.1585 PMid:32031570 PMCid:PMC7042881
- Xu X, Wu X, Jiang X, Xu K, Ying L, et al. Clinical findings in a group of patients infected with the 2019 novel coronavirus (SARS-Cov-2) outside of Wuhan, China: retrospective case series. BMJ, 2020;p.m606. https://doi.org/10.1136/bmj.m606 PMid:32075786
- Wu C, Chen X, Cai Y, Xia J, Zhou X, et al. Risk Factors Associated With Acute Respiratory Distress Syndrome and Death in Patients With Coronavirus Disease 2019 Pneumonia in Wuhan, China. JAMA Internal Medicine. 2020. https://doi.org/10.1001/jamainternmed.2020.0994 PMCid:PMC7070509
- Pan L, Mu M, Yang P, Sun Y, Wang R, et al. Clinical Characteristics of COVID-19 Patients With Digestive Symptoms in Hubei, China. The American Journal of Gastroenterology, 2020:1. https://doi.org/10.14309/ajg.0000000000000620 PMid:32287140 PMCid:PMC7172492
- [Internet]. Who.int. 2020 [cited 13 April 2020]. Available at: https://www.who.int/docs/default-source/searo/timor-leste/11-02-2020-tls-sitrep-7-ncov-final.pdf?sfvrsn=72720e51_2
- China’s National Health Commission news conference on coronavirus [Internet]. Aljazeera.com. 2020 [cited 13 April 2020]. Available at: https://www.aljazeera.com/news/2020/01/chinas-national-health-commission-news-conference-coronavirus-200126105935024.html
- How Coronavirus Symptoms Start, And What You Can Expect Each Day [Internet]. IFLScience. 2020 [cited 13 April 2020]. Available at: https://www.iflscience.com/health-and-medicine/a-daybyday-breakdown-of-coronavirus-symptoms-shows-how-the-disease-covid19-goes-from-bad-to-worse/
- A day-by-day breakdown of coronavirus symptoms shows how the disease COVID-19 goes from bad to worse [Internet]. Business Insider. 2020 [cited 13 April 2020]. Available at: https://www.businessinsider.com/coronavirus-covid19-day-by-day-symptoms-patients-2020-2
- What to do if you are sick with 2019 Novel Coronavirus. Available at: https://www.cdc.gov/coronavirus/2019-ncov/prevent-getting-sick/prevention.html
- World Health Organization, Novel Coronavirus (2019-nCoV) Advice for the Public, (2020). Available at: https://www.who.int/emergencies/diseases/novel-coronavirus-2019/advice-for-public
- Infection prevention and control during health care when COVID-19 is suspected. Available at: https://www.who.int/publications-detail/infection-prevention-and-control-during-health-care-when-novel-coronavirus-(ncov)-infection-is-suspected-20200125
- Chen X, Shang Y, Yao S, Liu R, Liu H. Perioperative care provider’s considerations in managing patients with the COVID-19 infections. Transl Perioper Pain Med. 2020;7:216-23. https://doi.org/10.31480/2330-4871/116
- Infection Prevention and Control for the safe management of a dead body in the context of COVID-19. Available at: https://apps.who.int/iris/bitstream/handle/10665/331538/WHO-COVID-19-lPC_DBMgmt-2020.1-eng.pdf?fbclid=IwAR1h_FQaIFcghLAGfFdWoCogtNzVcDUGtFLcfTjLqUUZ4IsYiiRE8k-I40A
- Characteristics of Infectious Disease | Microbiology. Available at: https://courses.lumenlearning.com/microbiology/chapter/characteristics-of-infectious-disease/
- Backer JA, Klinkenberg D, Wallinga J. Incubation period of 2019 novel coronavirus (2019-nCoV) infections among travellers from Wuhan, China, 20–28 January 2020. Eurosurveillance. 2020 Feb 6;25(5):2000062. https://doi.org/10.2807/1560-7917.ES.2020.25.5.2000062 PMid:32046819 PMCid:PMC7014672
- Linton NM, Kobayashi T, Yang Y, Hayashi K, Akhmetzhanov AR, Jung SM, et al. Incubation period and other epidemiological characteristics of 2019 novel coronavirus infections with right truncation: a statistical analysis of publicly available case data. Journal of clinical medicine. 2020 Feb;9(2):538. https://doi.org/10.3390/jcm9020538 PMid:32079150 PMCid:PMC7074197
- Lauer SA, Grantz KH, Bi Q, Jones FK, Zheng Q, Meredith HR, et al. The incubation period of coronavirus disease 2019 (COVID-19) from publicly reported confirmed cases: estimation and application. Annals of internal medicine. 2020 Mar 10. https://doi.org/10.7326/M20-0504 PMid:32150748 PMCid:PMC7081172
- Q&A on coronaviruses (COVID-19) [Internet]. Who.int. 2020 [cited 14 April 2020]. Available at: https://www.who.int/news-room/q-a-detail/q-a-coronaviruses
- Coronavirus Disease 2019 (COVID-19) [Internet]. Centers for Disease Control and Prevention. 2020 [cited 14 April 2020]. Available at: https://www.cdc.gov/coronavirus/2019-ncov/faq.html
- Department of Health | 2.2 The reproduction number [Internet]. www1.health.gov.au. 2020 [cited 14 April 2020]. Available at: https://www1.health.gov.au/internet/publications/publishing.nsf/Content/mathematical-models~mathematical-models-models.htm~mathematical-models-2.2.htm
- Epidemic theory (effective & basic reproduction numbers, epidemic thresholds) & techniques for analysis of infectious disease data (construction & use of epidemic curves, generation numbers, exceptional reporting & identification of significant clusters) [Internet]. Health Knowledge. 2020 [cited 14 April 2020]. Available at: https://www.healthknowledge.org.uk/public-health-textbook/research-methods/1a-epidemiology/epidemic-theory
- Rothman KJ, Lash T, Greenland S. Modern Epidemiology (3rd ed.), Lippincott Williams & Wilkins, 2013.
- Dietz K. The estimation of the basic reproduction number for infectious diseases. Statistical methods in medical research. 1993 Mar;2(1):23-41. https://doi.org/10.1177/096228029300200103 PMid:8261248
- Guerra FM, Bolotin S, Lim G, Heffernan J, Deeks SL, Li Y, Crowcroft NS. The basic reproduction number (R0) of measles: a systematic review. The Lancet Infectious Diseases. 2017 Dec 1;17(12):e420-8. https://doi.org/10.1016/S1473-3099(17)30307-9
- Zhao S, Lin Q, Ran J, Musa S, Yang G, Wang W, et al. Preliminary estimation of the basic reproduction number of novel coronavirus (2019-nCoV) in China, from 2019 to 2020: A data-driven analysis in the early phase of the outbreak. International Journal of Infectious Diseases. 2020;92:214-7. https://doi.org/10.1016/j.ijid.2020.01.050 PMid:32007643 PMCid:PMC7110798
- Cowling B, Fang V, Riley S, Malik Peiris J, Leung G. Estimation of the Serial Interval of Influenza. Epidemiology. 2009;20(3):344-7. https://doi.org/10.1097/EDE.0b013e31819d1092 PMid:19279492 PMCid:PMC3057478
- Wu JT, Leung K, Leung GM. Nowcasting and forecasting the potential domestic and international spread of the 2019-nCoV outbreak originating in Wuhan, China: a modelling study. The Lancet. 2020 Feb 29;395(10225):689-97. https://doi.org/10.1016/S0140-6736(20)30260-9
- Li Q, Guan X, Wu P, Wang X, Zhou L, Tong Y, et al. Early transmission dynamics in Wuhan, China, of novel coronavirus–infected pneumonia. New England Journal of Medicine. 2020 Jan 29. Available at: https://www.nejm.org/doi/full/10.1056/NEJMoa2001316
- Perlman S. Another Decade, Another Coronavirus. New England Journal of Medicine. 2020;382(8):760-2. https://doi.org/10.1056/NEJMe2001126 PMid:31978944 PMCid:PMC7121143
- Sanche S, Lin Y, Xu C, Romero-Severson E, Hengartner N, Ke R. High Contagiousness and Rapid Spread of Severe Acute Respiratory Syndrome Coronavirus 2. Emerging Infectious Diseases. 2020;26(7). https://doi.org/10.3201/eid2607.200282 PMid:32255761
- Bi Q, Wu Y, Mei S, Ye C, Zou X, Zhang Z, et al. Epidemiology and Transmission of COVID-19 in Shenzhen China: Analysis of 391 cases and 1,286 of their close contacts. 2020. https://doi.org/10.1101/2020.03.03.20028423
- Liu Y, Gayle A, Wilder-Smith A, Rocklöv J. The reproductive number of COVID-19 is higher compared to SARS coronavirus. Journal of Travel Medicine. 2020;27(2). https://doi.org/10.1093/jtm/taaa021 PMid:32052846 PMCid:PMC7074654
- World Health Organization. Report of the WHO-China joint mission on coronavirus disease 2019 (covid-19). Available at: https://www.who.int/publications-detail/report-of-the-who-china-joint-mission-on-coronavirus-disease-2019-(covid-19)
- Peeri N, Shrestha N, Rahman M, Zaki R, Tan Z, Bibi S, et al. The SARS, MERS and novel coronavirus (COVID-19) epidemics, the newest and biggest global health threats: what lessons have we learned?. International Journal of Epidemiology. 2020. https://doi.org/10.1093/ije/dyaa033 PMid:32086938 PMCid:PMC7197734
- Virlogeux V, Fang VJ, Park M, Wu JT, Cowling BJ. Comparison of incubation period distribution of human infections with MERS-CoV in South Korea and Saudi Arabia. Scientific reports. 2016 Oct 24;6:35839. https://doi.org/10.1038/srep35839 PMid:27775012 PMCid:PMC5075793
- Frequently asked questions on Middle East respiratory syndrome coronavirus. Available at: https://www.who.int/csr/disease/coronavirus_infections/faq/en/
- Chowell G, Blumberg S, Simonsen L, Miller MA, Viboud C. Synthesizing data and models for the spread of MERS-CoV, 2013: key role of index cases and hospital transmission. Epidemics. 2014 Dec 1;9:40-51. https://doi.org/10.1016/j.epidem.2014.09.011 PMid:25480133 PMCid:PMC4258236
- Alenazi TH, Al Arbash H, El-Saed A, Alshamrani MM, Baffoe-Bonnie H, Arabi YM, et al. Identified transmission dynamics of Middle East respiratory syndrome coronavirus infection during an outbreak: implications of an overcrowded emergency department. Clinical Infectious Diseases. 2017 Aug 15;65(4):675-9. https://doi.org/10.1093/cid/cix352 PMid:28575307 PMCid:PMC7108118
- Eifan SA, Nour I, Hanif A, Zamzam AM, AlJohani SM. A pandemic risk assessment of Middle East respiratory syndrome coronavirus (MERS-CoV) in Saudi Arabia. Saudi journal of biological sciences. 2017 Nov 1;24(7):1631-8. https://doi.org/10.1016/j.sjbs.2017.06.001 PMid:29062261 PMCid:PMC5643837
- Poletto C, Pelat C, Lévy-Bruhl D, Yazdanpanah Y, Boëlle P, Colizza V. Assessment of the Middle East respiratory syndrome coronavirus (MERS-CoV) epidemic in the Middle East and risk of international spread using a novel maximum likelihood analysis approach. Eurosurveillance. 2014;19(23). https://doi.org/10.2807/1560-7917.ES2014.19.23.20824
- Kim Y, Lee S, Chu C, Choe S, Hong S, Shin Y. The characteristics of Middle Eastern respiratory syndrome coronavirus transmission dynamics in South Korea. Osong public health and research perspectives. 2016 Feb 1;7(1):49-55. https://doi.org/10.1016/j.phrp.2016.01.001 PMid:26981343 PMCid:PMC4776270
- Zhang XS, Pebody R, Charlett A, de Angelis D, Birrell P, Kang H, et al. Estimating and modelling the transmissibility of Middle East Respiratory Syndrome CoronaVirus during the 2015 outbreak in the Republic of Korea. Influenza and other respiratory viruses. 2017 Sep;11(5):434-44. https://doi.org/10.1111/irv.12467 PMid: 28703921 PMCid:PMC5598245
- Chang HJ. Estimation of basic reproduction number of the Middle East respiratory syndrome coronavirus (MERS-CoV) during the outbreak in South Korea, 2015. Biomedical engineering online. 2017 Dec 1;16(1):79. https://doi.org/10.1186/s12938-017-0370-7 PMid:28610609 PMCid:PMC5470331
- Xia ZQ, Zhang J, Xue YK, Sun GQ, Jin Z. Modeling the transmission of Middle East respirator syndrome corona virus in the Republic of Korea. PloS one. 2015;10(12). https://doi.org/10.1371/journal.pone.0144778 PMid:26690750 PMCid:PMC4686901
- Middle East respiratory syndrome coronavirus (MERS-CoV) [Internet]. World Health Organization. 2020 [cited 15 April 2020]. Available at: https://www.who.int/emergencies/mers-cov/en/
- WHO | SARS (Severe Acute Respiratory Syndrome) [Internet]. Who.int. 2020 [cited 15 April 2020]. Available at: https://www.who.int/ith/diseases/sars/en/
- [Internet]. Who.int. 2020 [cited 15 April 2020]. Available from: https://www.who.int/csr/sars/en/WHOconsensus.pdf
- Lipsitch M, Cohen T, Cooper B, Robins JM, Ma S, James L, et al. Transmission dynamics and control of severe acute respiratory syndrome. Science. 2003 Jun 20;300(5627):1966-70. https://doi.org/10.1126/science.1086616 PMid:12766207 PMCid:PMC2760158
- WHO | Recommended procedures for prevention and management of probable cases of SARS on International Cruise Vessels [Internet]. Who.int. 2020 [cited 15 April 2020]. Available at: https://www.who.int/csr/sars/travel/vessels/en/
- Division U. UNSD — Methodology [Internet]. Unstats.un.org. 2020 [cited 15 April 2020]. Available at: https://unstats.un.org/unsd/methodology/m49/
- Ebola virus disease [Internet]. Who.int. 2020 [cited 15 April 2020]. Available at: https://www.who.int/news-room/fact-sheets/detail/ebola-virus-disease
- Althaus CL. Estimating the reproduction number of Ebola virus (EBOV) during the 2014 outbreak in West Africa. PLoS currents. 2014 Sep 2;6. https://doi.org/10.1371/currents.outbreaks.91afb5e0f279e7f29e7056095255b288
- 2014-2016 Ebola Outbreak in West Africa | History | Ebola (Ebola Virus Disease) | CDC [Internet]. Cdc.gov. 2020 [cited 15 April 2020]. Available at: https://www.cdc.gov/vhf/ebola/history/2014-2016-outbreak/index.html
- COVID-19. The importance of diagnostic testing for COVID-19 [Internet]. Infectious Diseases Hub. 2020 [cited 17 April 2020]. Available at: https://www.id-hub.com/2020/04/02/the-importance-of-diagnostic-testing-for-covid-19/
- Diseases S. COVID-19 science: Why testing is so important [Internet]. Medicalxpress.com. 2020 [cited 17 April 2020]. Available at: https://medicalxpress.com/news/2020-04-covid-science-important.html
- Correction to Lancet Glob Health 2020; published online Feb 28. The Lancet Global Health. 2020. https://doi.org/10.1016/S2214-109X(20)30074-7
- To K, Tsang O, Yip C, Chan K, Wu T, Chan J, et al. Consistent Detection of 2019 Novel Coronavirus in Saliva. Clinical Infectious Diseases. 2020. https://doi.org/10.1093/cid/ciaa149
- To KK, Yip CC, Lai CY, Wong CK, Ho DT, Pang PK, et al. Saliva as a diagnostic specimen for testing respiratory virus by a point-of-care molecular assay: a diagnostic validity study. Clinical Microbiology & Infection. 2019 Mar 1;25(3):372-8. https://doi.org/10.1016/j.cmi.2018.06.009 PMid:29906597
- Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Eurosurveillance. 2020 Jan 23;25(3):2000045. https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045
- Li X, Geng M, Peng Y, Meng L, Lu S. Molecular immune pathogenesis and diagnosis of COVID-19. Journal of Pharmaceutical Analysis. 2020 Mar 5. https://doi.org/10.1016/j.jpha.2020.03.001
- Xie C, Jiang L, Huang G, Pu H, Gong B, Lin H, et al. Comparison of different samples for 2019 novel coronavirus detection by nucleic acid amplification tests. International Journal of Infectious Diseases. 2020 Feb 27. https://doi.org/10.1016/j.ijid.2020.02.050 PMid:32114193 PMCid:PMC7129110
- Yam WC, Chan KH, Poon LL, Guan Y, Yuen KY, Seto WH, Peiris JS. Evaluation of reverse transcription-PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus. Journal of clinical microbiology. 2003 Oct 1;41(10):4521-4. https://doi.org/10.1128/JCM.41.10.4521-4524.2003 PMid:14532176 PMCid:PMC254368
- Administrator J. Global Progress on COVID-19 Serology-Based Testing [Internet]. Johns Hopkins Center for Health Security. 2020 [cited 17 April 2020]. Available at: https://www.centerforhealthsecurity.org/resources/COVID-19/serology/Serology-based-tests-for-COVID-19.html
- Liu W, Liu L, Kou G, Zheng Y, Ding Y, Ni W, et al. Evaluation of Nucleocapsid and Spike Protein-based ELISAs for detecting antibodies against SARS-CoV-2. Journal of Clinical Microbiology. 2020 Mar 30. https://doi.org/10.1128/JCM.00461-20 PMid:32229605
- Li Z, Yi Y, Luo X, Xiong N, Liu Y, Li S, et al. Development and clinical application of a rapid IgM‐IgG combined antibody test for SARS‐CoV‐2 infection diagnosis. Journal of medical virology. 2020 Feb 27. https://doi.org/10.1002/jmv.25727
- Why Do We Need Antibody Tests for COVID-19 and How to Interpret Test Results [Internet]. Diazyme Laboratories. 2020 [cited 17 April 2020]. Available at: http://www.diazyme.com/covid-19-antibody-tests
- Pan Y, Guan H, Zhou S, Wang Y, Li Q, Zhu T, et al. Initial CT findings and temporal changes in patients with the novel coronavirus pneumonia (2019-nCoV): a study of 63 patients in Wuhan, China. European Radiology. 2020. https://doi.org/10.1007/s00330-020-06731-x
- Kanne JP. Chest CT findings in 2019 novel coronavirus (2019-nCoV) infections from Wuhan, China: key points for the radiologist. https://doi.org/10.1148/radiol.2020200241
- Müller NL, Ooi GC, Khong PL, Zhou LJ, Tsang KW, Nicolaou S. High-resolution CT findings of severe acute respiratory syndrome at presentation and after admission. American Journal of Roentgenology. 2004 Jan;182(1):39-44. https://doi.org/10.2214/ajr.182.1.1820039 PMid:14684509
- Das KM, Lee EY, Jawder SE, Enani MA, Singh R, Skakni L, et al. Acute Middle East respiratory syndrome coronavirus: temporal lung changes observed on the chest radiographs of 55 patients. American Journal of Roentgenology. 2015 Sep;205(3):W267-S274. https://doi.org/10.2214/AJR.15.14445 PMid:26102309
- Chung M, Bernheim A, Mei X, Zhang N, Huang M, Zeng X, et al. CT imaging features of 2019 novel coronavirus (2019-nCoV). Radiology. 2020 Apr;295(1):202-7. https://doi.org/10.1148/radiol.2020200230 PMid:32017661
- Ullah MA, Araf Y, Sarkar B, Moin AT, Reshad RAI, Rahman MH. Pathogenesis, Diagnosis and Possible Therapeutic Options for COVID-19. Preprints 2020, 2020040372. https://doi.org/10.20944/preprints202004.0372.v1
- Zhang JJ, Dong X, Cao YY, Yuan YD, Yang YB, Yan YQ, et al. Clinical characteristics of 140 patients infected with SARS‐CoV‐2 in Wuhan, China. Allergy. 2020 Feb 19. https://doi.org/10.1111/all.14238 PMid:32077115
- Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, et al. CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. 2020 Apr 16:1-5. https://doi.org/10.1038/s41587-020-0513-4 PMid:32300245
- Gene editing by CRISPR–Cas [Internet]. Nature.com. 2020 [cited 22 April 2020]. Available at: https://www.nature.com/articles/d42859-019-00088-y
- Contributor A. What Is CRISPR? [Internet]. livescience.com. 2020 [cited 22 April 2020]. Available at: https://www.livescience.com/58790-crispr-explained.html
- Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA. CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Science. 2018 Apr 27;360(6387):436-9. https://doi.org/10.1126/science.aar6245 PMid:29449511 PMCid:PMC6628903
- Li SY, Cheng QX, Wang JM, Li XY, Zhang ZL, Gao S, et al. CRISPR-Cas12a-assisted nucleic acid detection. Cell discovery. 2018 Apr 24;4(1):1-4. https://doi.org/10.1038/s41421-018-0028-z PMid:29707234 PMCid:PMC5913299
- Coronavirus Disease 2019 (COVID-19) [Internet]. Centers for Disease Control and Prevention. 2020 [cited 22 April 2020]. Available at: https://www.cdc.gov/coronavirus/2019-ncov/lab/index.html?CDC_AA_refVal=https%3A%2F%2Fwww.cdc.gov%2Fcoronavirus%2F2019-ncov%2Flab%2Frt-pcr-detection-instructions.html
- [Internet]. Who.int. 2020 [cited 22 April 2020]. Available at: https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf
- [Internet]. Fda.gov. 2020 [cited 22 April 2020]. Available at: https://www.fda.gov/media/136315/download
- Myhrvold C, Freije CA, Gootenberg JS, Abudayyeh OO, Metsky HC, Durbin AF, et al. Field-deployable viral diagnostics using CRISPR-Cas13. Science. 2018 Apr 27;360(6387):444-8. https://doi.org/10.1126/science.aas8836 PMid:29700266 PMCid:PMC6197056
- Zhang F, Abudayyeh OO, Gootenberg JS. A protocol for detection of COVID-19 using CRISPR diagnostics. A protocol for detection of COVID-19 using CRISPR diagnostics. 2020;8. Available at: https://go.idtdna.com/rs/400-UEU-432/images/Zhang%20et%20al.%2C%202020%20COVID-19%20detection%20%28updated%29.pdf
- Wang LS, Wang YR, Ye DW, Liu QQ. A review of the 2019 Novel Coronavirus (COVID-19) based on current evidence. International journal of antimicrobial agents. 2020 Mar 19:105948. https://doi.org/10.1016/j.ijantimicag.2020.105948
- Wu D, Wu T, Liu Q, Yang Z. The SARS-CoV-2 outbreak: What we know. International Journal of Infectious Diseases. 2020;94. https://doi.org/10.1016/j.ijid.2020.03.004 PMid: 32171952 PMCid:PMC7102543
- Al-Quteimat OM, Amer AM. SARS-CoV-2 outbreak: How can pharmacists help? Research in Social and Administrative Pharmacy. 2020. https://doi.org/10.1016/j.sapharm.2020.03.018 PMid:32241695
- Zumla A, Chan JFW, Azhar EI, Hui DSC, Yuen K-Y. Coronaviruses — drug discovery and therapeutic options. Nature Reviews Drug Discovery. 2016Dec;15(5). https://doi.org/10.1038/nrd.2015.37 PMid:26868298 PMCid:PMC7097181
- Jin Y-H, Cai L, Cheng Z-S, Cheng H, Deng T, Fan Y-P, et al. A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus (2019-nCoV) infected pneumonia (standard version). Military Medical Research. 2020Jun;7(1). https://doi.org/10.1186/s40779-020-00246-8
- FIP Covid-19 Information Hub [Internet]. fip. [cited 2020Apr24]. Available at: https://www.fip.org/coronavirus
- NHS Choices. NHS; [cited 2020Apr24]. Available at: https://www.england.nhs.uk/coronavirus/publication/novel-coronavirus-covid-19-standard-operating-procedure-community-health-services/
- Lu H. Drug treatment options for the 2019-new coronavirus (2019-nCoV). BioScience Trends. 2020;14(1). https://doi.org/10.5582/bst.2020.01020 PMid:31996494
- Wu Z, Mcgoogan JM. Characteristics of and Important Lessons From the Coronavirus Disease 2019 (COVID-19) Outbreak in China. Jama. 2020Jul;323(13). https://doi.org/10.1001/jama.2020.2648 PMid:32091533
- Kim Y, Liu H, Kankanamalage ACG, Weerasekara S, Hua DH, Groutas WC, et al. Reversal of the Progression of Fatal Coronavirus Infection in Cats by a Broad-Spectrum Coronavirus Protease Inhibitor. PLOS Pathogens. 2016;12(3). https://doi.org/10.1371/journal.ppat.1005531 PMid:27027316 PMCid:PMC4814111
- Channappanavar R, Fett C, Mack M, Eyck PPT, Meyerholz DK, Perlman S. Sex-Based Differences in Susceptibility to Severe Acute Respiratory Syndrome Coronavirus Infection. The Journal of Immunology. 2017Mar;198(10). https://doi.org/10.4049/jimmunol.1601896 PMid:28373583 PMCid:PMC5450662
- Vellingiri B, Jayaramayya K, Iyer M, Narayanasamy A, Govindasamy V, Giridharan B, et al. COVID-19: A promising cure for the global panic. Science of The Total Environment. 2020;725. https://doi.org/10.1016/j.scitotenv.2020.138277 PMid:32278175 PMCid:PMC7128376
- Mulangu S, Dodd LE, Davey RT, Mbaya OT, Proschan M, Mukadi D, et al. A Randomized, Controlled Trial of Ebola Virus Disease Therapeutics. New England Journal of Medicine. 2019Dec;381(24). https://doi.org/10.1056/NEJMoa1910993 PMid:31774950
- Tchesnokov E, Feng J, Porter D, Götte M. Mechanism of Inhibition of Ebola Virus RNA-Dependent RNA Polymerase by Remdesivir. Viruses. 2019Apr;11(4). https://doi.org/10.3390/v11040326 PMid:30987343 PMCid:PMC6520719
- Agostini ML, Andres EL, Sims AC, Graham RL, Sheahan TP, Lu X, et al. Coronavirus Susceptibility to the Antiviral Remdesivir (GS-5734) Is Mediated by the Viral Polymerase and the Proofreading Exoribonuclease. mBio. 2018Jun;9(2). https://doi.org/10.1128/mBio.00221-18 PMid:29511076 PMCid:PMC5844999
- Warren TK, Jordan R, Lo MK, Ray AS, Mackman RL, Soloveva V, et al. Therapeutic efficacy of the small molecule GS-5734 against Ebola virus in rhesus monkeys. Nature. 2016 Mar;531(7594). https://doi.org/10.1038/nature17180
- Arabi YM, Alothman A, Balkhy HH, Al-Dawood A, Aljohani S, Harbi SA, et al. Treatment of Middle East Respiratory Syndrome with a combination of lopinavir-ritonavir and interferon-β1b (MIRACLE trial): study protocol for a randomized controlled trial. Trials. 2018;19(1). https://doi.org/10.1186/s13063-017-2427-0 PMid:29382391 PMCid:PMC5791210
- Sheahan TP, Sims AC, Graham RL, Menachery VD, Gralinski LE, Case JB, et al. Broad-spectrum antiviral GS-5734 inhibits both epidemic and zoonotic coronaviruses. Science Translational Medicine. 2017;9(396). https://doi.org/10.1126/scitranslmed.aal3653 PMid:28659436 PMCid:PMC5567817
- Menachery VD, Yount BL, Debbink K, Agnihothram S, Gralinski LE, Plante JA, et al. A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence. Nature Medicine. 2015Sep;21(12). https://doi.org/10.1038/nm.3985 PMid:26552008 PMCid:PMC4797993
- Brown AJ, Won JJ, Graham RL, Dinnon KH, Sims AC, Feng JY, et al. Broad spectrum antiviral remdesivir inhibits human endemic and zoonotic deltacoronaviruses with a highly divergent RNA dependent RNA polymerase. Antiviral Research. 2019;169. https://doi.org/10.1016/j.antiviral.2019.104541 PMid:31233808 PMCid:PMC6699884
- de Wit E, Feldmann F, Cronin J, Jordan R, Okumura A, Thomas T, et al. Prophylactic and therapeutic remdesivir (GS-5734) treatment in the rhesus macaque model of MERS-CoV infection. Proceedings of the National Academy of Sciences. 2020 Mar 24;117(12). https://doi.org/10.1073/pnas.1922083117 PMid:32054787 PMCid:PMC7104368
- Wang M, Cao R, Zhang L, Yang X, Liu J, Xu M, et al. Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro. Cell Research. 2020Apr;30(3). https://doi.org/10.1038/s41422-020-0282-0 PMid:32020029 PMCid:PMC7054408
- Xiao F, Tang M, Zheng X, Liu Y, Li X, Shan H. Evidence for gastrointestinal infection of SARS-CoV-2. Gastroenterology. 2020 Mar 3. https://doi.org/10.1101/2020.02.17.20023721
- Stebbing J, Phelan A, Griffin I, Tucker C, Oechsle O, Smith D, et al. COVID-19: combining antiviral and anti-inflammatory treatments. The Lancet Infectious Diseases. 2020;20(4). https://doi.org/10.1016/S1473-3099(20)30132-8
- Lai C-C, Shih T-P, Ko W-C, Tang H-J, Hsueh P-R. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and coronavirus disease-2019 (COVID-19): The epidemic and the challenges. International Journal of Antimicrobial Agents. 2020;55(3). https://doi.org/10.1016/j.ijantimicag.2020.105924 PMid:32081636 PMCid:PMC7127800
- Zhai P, Ding Y, Wu X, Long J, Zhong Y, Li Y. The epidemiology, diagnosis and treatment of COVID-19. International Journal of Antimicrobial Agents. 2020. https://doi.org/10.1016/j.ijantimicag.2020.105955 PMid:32234468 PMCid:PMC7138178
- Falzarano D, Wit ED, Rasmussen AL, Feldmann F, Okumura A, Scott DP, et al. Treatment with interferon-α2b and ribavirin improves outcome in MERS-CoV–infected rhesus macaques. Nature Medicine. 2013Aug;19(10). https://doi.org/10.1038/nm.3362 PMid:24013700 PMCid:PMC4093902
- Elfiky AA. Anti-HCV, nucleotide inhibitors, repurposing against COVID-19. Life Sciences. 2020;248. https://doi.org/10.1016/j.lfs.2020.117477 PMid:32119961 PMCid:PMC7089605
- Wang Z, Chen X, Lu Y, Chen F, Zhang W. Clinical characteristics and therapeutic procedure for four cases with 2019 novel coronavirus pneumonia receiving combined Chinese and Western medicine treatment. BioScience Trends. 2020;14(1). https://doi.org/10.5582/bst.2020.01030 PMid:32037389
- Chu CM, Cheng VC, Hung IF, Wong MM, Chan KH, Chan KS, et al. Role of lopinavir/ritonavir in the treatment of SARS: initial virological and clinical findings. Thorax. 2004 Mar 1;59(3):252-6. https://doi.org/10.1136/thorax.2003.012658 PMid:14985565 PMCid:PMC1746980
- Yao TT, Qian JD, Zhu WY, Wang Y, Wang GQ. A systematic review of lopinavir therapy for SARS coronavirus and MERS coronavirus—A possible reference for coronavirus disease‐19 treatment option. Journal of Medical Virology. 2020Dec;92(6). https://doi.org/10.1002/jmv.25729 PMid:32104907
- Wu C, Liu Y, Yang Y, Zhang P, Zhong W, Wang Y, et al. Analysis of therapeutic targets for SARS-CoV-2 and discovery of potential drugs by computational methods. Acta Pharmaceutica Sinica B. 2020. https://doi.org/10.1016/j.apsb.2020.02.008
- Morse JS, Lalonde T, Xu S, Liu WR. Learning from the Past: Possible Urgent Prevention and Treatment Options for Severe Acute Respiratory Infections Caused by 2019‐nCoV. ChemBioChem. 2020;21(5). https://doi.org/10.1002/cbic.202000047 PMid:32022370 PMCid:PMC7162020
- Savarino A, Di Trani L, Donatelli I, Cauda R, Cassone A. New insights into the antiviral effects of chloroquine. The Lancet infectious diseases. 2006 Feb 1;6(2):67-9. https://doi.org/10.1016/S1473-3099(06)70361-9
- Yan Y, Zou Z, Sun Y, Li X, Xu K-F, Wei Y, et al. Anti-malaria drug chloroquine is highly effective in treating avian influenza A H5N1 virus infection in an animal model. Cell Research. 2012Apr;23(2). https://doi.org/10.1038/cr.2012.165 PMid:23208422 PMCid:PMC3567830
- Rolain J-M, Colson P, Raoult D. Recycling of chloroquine and its hydroxyl analogue to face bacterial, fungal and viral infections in the 21st century. International Journal of Antimicrobial Agents. 2007;30(4). https://doi.org/10.1016/j.ijantimicag.2007.05.015 PMid:17629679 PMCid:PMC7126847
- Schrezenmeier E, Dörner T. Mechanisms of action of hydroxychloroquine and chloroquine: implications for rheumatology. Nature Reviews Rheumatology. 2020Jul;16(3). https://doi.org/10.1038/s41584-020-0372-x PMid:32034323
- Savarino A, Boelaert JR, Cassone A, Majori G, Cauda R. Effects of chloroquine on viral infections: an old drug against todays diseases. The Lancet Infectious Diseases. 2003;3(11). https://doi.org/10.1016/S1473-3099(03)00806-5
- Gao J, Tian Z, Yang X. Breakthrough: Chloroquine phosphate has shown apparent efficacy in treatment of COVID-19 associated pneumonia in clinical studies. BioScience Trends. 2020;14(1). https://doi.org/10.5582/bst.2020.01047 PMid:32074550
- Vincent MJ, Bergeron E, Benjannet S, Erickson BR, Rollin PE, Ksiazek TG, Seidah NG, Nichol ST. Chloroquine is a potent inhibitor of SARS coronavirus infection and spread. Virology journal. 2005 Dec 1;2(1):69. https://doi.org/10.1186/1743-422X-2-69 PMid:16115318 PMCid:PMC1232869
- Yao X, Ye F, Zhang M, Cui C, Huang B, Niu P, et al. In Vitro Antiviral Activity and Projection of Optimized Dosing Design of Hydroxychloroquine for the Treatment of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Clinical Infectious Diseases. 2020Sep. https://doi.org/10.1093/cid/ciaa237 PMid:32150618 PMCid:PMC7108130
- Jie Z, He H, Xi H, Zhi Z. Multicenter Collaboration Group of Department of Science and Technology of Guangdong Province and Health Commission of Guangdong Province for Chloroquine in the Treatment of Novel Coronavirus Pneumonia. Expert Consensus on Chloroquine Phosphate for the Treatment of Novel Coronavirus Pneumonia [in Chinese]. 2020;10:1001-0939.
- Colson P, Rolain J-M, Raoult D. Chloroquine for the 2019 novel coronavirus SARS-CoV-2. International Journal of Antimicrobial Agents. 2020;55(3). https://doi.org/10.1016/j.ijantimicag.2020.105923 PMid:32070753 PMCid:PMC7134866
- Mair-Jenkins J, Saavedra-Campos M, Baillie JK, Cleary P, Khaw F-M, Lim WS, et al. The Effectiveness of Convalescent Plasma and Hyperimmune Immunoglobulin for the Treatment of Severe Acute Respiratory Infections of Viral Etiology: A Systematic Review and Exploratory Meta-analysis. Journal of Infectious Diseases. 2014;211(1). https://doi.org/10.1093/infdis/jiu396 PMid:25030060 PMCid:PMC4264590
- Cheng Y, Wong R, Soo YOY, Wong WS, Lee CK, Ng MHL, et al. Use of convalescent plasma therapy in SARS patients in Hong Kong. European Journal of Clinical Microbiology & Infectious Diseases. 2004;24(1). https://doi.org/10.1007/s10096-004-1271-9 PMid:15616839 PMCid:PMC7088355
- Hung IF, To KK, Lee CK, Lee KL, Chan K, Yan WW, et al. Convalescent plasma treatment reduced mortality in patients with severe pandemic influenza A (H1N1) 2009 virus infection. Clinical Infectious Diseases. 2011 Feb 15;52(4). https://doi.org/10.1093/cid/ciq106 PMid: 21248066
- Baron S, editor. Medical Microbiology. 4th edition. Galveston (TX): University of Texas Medical Branch at Galveston; 1996. Available at: https://www.ncbi.nlm.nih.gov/books/NBK7627/
- Soo Y, Cheng Y, Wong R, Hui D, Lee C, Tsang K, et al. Retrospective comparison of convalescent plasma with continuing high-dose methylprednisolone treatment in SARS patients. Clinical Microbiology and Infection. 2004;10(7). https://doi.org/10.1111/j.1469-0691.2004.00956.x PMid:15214887 PMCid:PMC7129386
- Du L, He Y, Zhou Y, Liu S, Zheng BJ, Jiang S. The spike protein of SARS-CoV—a target for vaccine and therapeutic development. Nature Reviews Microbiology. 2009 Mar;7(3). https://doi.org/10.1038/nrmicro2090 PMid: 19198616 PMCid:PMC2750777
- Zhang J, Zeng H, Gu J, Li H, Zheng L, Zou Q. Progress and Prospects on Vaccine Development against SARS-CoV-2. Vaccines. 2020 Jun;8(2). https://doi.org/10.3390/vaccines8020153 PMid:32235387
- Agnandji ST, Huttner A, Zinser ME, Njuguna P, Dahlke C, Fernandes JF, et al. Phase 1 trials of rVSV Ebola vaccine in Africa and Europe. New England Journal of Medicine. 2016 Apr 28;374(17). Available at: https://www.nejm.org/doi/full/10.1056/nejmoa1502924
- Diseases TLI. Challenges of coronavirus disease 2019. The Lancet Infectious Diseases. 2020;20(3). https://doi.org/10.1016/S1473-3099(20)30072-4
- Amanat F, Krammer F. SARS-CoV-2 vaccines: status report. Immunity. 2020 Apr 6. https://doi.org/10.1016/j.immuni.2020.03.007 PMid:32259480 PMCid:PMC7136867
- Sambhara S, Mcelhaney JE. Immunosenescence and Influenza Vaccine Efficacy. Current Topics in Microbiology and Immunology Vaccines for Pandemic Influenza. 2009. https://doi.org/10.1007/978-3-540-92165-3_20 PMid:19768417 PMCid:PMC7121450
- Sharma A, Krause A, Worgall S. Recent developments for Pseudomonas vaccines. Human Vaccines. 2011;7(10). https://doi.org/10.4161/hv.7.10.16369 PMid:21941090 PMCid:PMC3360073
- Le TT, Andreadakis Z, Kumar A, Román RG, Tollefsen S, Saville M, et al. The COVID-19 vaccine development landscape [Internet]. Nature News. Nature Publishing Group; 2020 [cited 2020Apr15]. Available at: https://www.nature.com/articles/d41573-020-00073-5
- Pardi N, Hogan MJ, Porter FW, Weissman D. mRNA vaccines — a new era in vaccinology. Nature Reviews Drug Discovery. 2018Dec;17(4). https://doi.org/10.1038/nrd.2017.243 PMid:29326426 PMCid:PMC5906799
- Gonzalez-Nicolini V, Sanchez-Bustamante CD, Hartenbach S, Fussenegger M. Adenoviral vector platform for transduction of constitutive and regulated tricistronic or triple-transcript transgene expression in mammalian cells and microtissues. The Journal of Gene Medicine. 2006;8(10). https://doi.org/10.1002/jgm.960 PMid: 16960915
- Azmi F, Fuaad AAHA, Skwarczynski M, Toth I. Recent progress in adjuvant discovery for peptide-based subunit vaccines. Human Vaccines & Immunotherapeutics. 2013Mar;10(3). https://doi.org/10.4161/hv.27332 PMid: 24300669 PMCid:PMC4130256
- Ahmed SF, Quadeer AA, Mckay MR. Preliminary identification of potential vaccine targets for the COVID-19 coronavirus (SARS-CoV-2) based on SARS-CoV immunological studies. 2020Apr. https://doi.org/10.1101/2020.02.03.933226
- Liu H, Su D, Zhang J, Ge S, Li Y, Wang F, et al. Improvement of Pharmacokinetic Profile of TRAIL via Trimer-Tag Enhances its Antitumor Activity in vivo. Scientific Reports. 2017;7(1). https://doi.org/10.1038/s41598-017-09518-1 PMid:28827692 PMCid:PMC5566391
- Takashima Y, Osaki M, Ishimaru Y, Yamaguchi H, Harada A. Artificial molecular clamp: a novel device for synthetic polymerases. Angewandte Chemie International Edition. 2011 Aug 8;50(33):7524-8. https://doi.org/10.1002/anie.201102834
- Walsh F. Coronavirus: First patients injected in UK vaccine trial [Internet]. BBC News. BBC; 2020 [cited 2020Apr27]. Available at: https://www.bbc.com/news/health-52394485
- Lane R. Sarah Gilbert: carving a path towards a COVID-19 vaccine. The Lancet. 2020;395(10232). https://doi.org/10.1016/S0140-6736(20)30796-0
- Decaro N, Lorusso A. Novel human coronavirus (SARS-CoV-2): a lesson from animal coronaviruses. Veterinary Microbiology. 2020 Apr 14:108693. https://doi.org/10.1016/j.vetmic.2020.108693 PMCid:PMC7195271
- Li H, Mendelsohn E, Zong C, Zhang W, Hagan E, Wang N, et al. Human-animal interactions and bat coronavirus spillover potential among rural residents in Southern China. Biosafety and Health. 2019 Sep 1;1(2). https://doi.org/10.1016/j.bsheal.2019.10.004 PMCid:PMC7148670
- Aljofan M, Gaipov A. COVID-19 Treatment: The Race Against Time. Electronic Journal of General Medicine. 2020May;17(6). https://doi.org/10.29333/ejgm/7890
- Zhao H, Wald J, Palmer M, Han Y. Hydroxychloroquine-induced cardiomyopathy and heart failure in twins. Journal of Thoracic Disease. 2018;10(1). https://doi.org/10.21037/jtd.2017.12.66 PMid:29600108 PMCid:PMC5863196
- Sebba A. Tocilizumab: The first interleukin-6-receptor inhibitor. American Journal of Health-System Pharmacy. 2008Jan;65(15). https://doi.org/10.2146/ajhp070449 PMid:18653811
- Shimabukuro-Vornhagen A, Gödel P, Subklewe M, Stemmler HJ, Schlößer HA, Schlaak M, et al. Cytokine release syndrome. Journal for ImmunoTherapy of Cancer. 2018;6(1). https://doi.org/10.1186/s40425-018-0343-9 PMid:29907163 PMCid:PMC6003181
- Russell CD, Millar JE, Baillie JK. Clinical evidence does not support corticosteroid treatment for 2019-nCoV lung injury. The Lancet. 2020;395(10223). https://doi.org/10.1016/S0140-6736(20)30317-2
- Chinese Clinical Trial Register (ChiCTR) - The world health organization international clinical trials registered organization registered platform. [cited 2020May6]. Available at: http://www.chictr.org.cn/showprojen.aspx?proj=49409
- Sharquie IK. BCG is a Good Immunotherapeutic Agent for Viral and Autoimmune Diseases: Is it a New Weapon against Coronavirus (COVID-19)? Electronic Journal of General Medicine. 2020Jun;17(6). https://doi.org/10.29333/ejgm/7892
- Coronavirus FAQ’s by Dr. Megan ... - Abundance Foundation [Internet]. [cited 2020May6]. Available at: https://www.abundance.org/coronavirus-faqs-by-dr-megan-murray-harvard-infectious-disease-specialist/
- Singh R, Mahajan B, Dhawan M. Herpes genitalis - Topical zinc sulfate: An alternative therapeutic and modality. Indian Journal of Sexually Transmitted Diseases and AIDS. 2013;34(1). ttps://doi.org/10.4103/0253-7184.112867 PMid:23919052 PMCid:PMC3730471
- Al-Gurairi F, Al-Waiz M, Sharquie K. Oral zinc sulphate in the treatment of recalcitrant viral warts: randomized placebo-controlled clinical trial. British Journal of Dermatology. 2002;146(3). https://doi.org/10.1046/j.1365-2133.2002.04617.x PMid:11952542
- Sharquie KE, Noaimi AA, Al-Salam WS. Treatment of Acute Cutaneous Leishmaniasis by Oral Zinc Sulfate and Oral Ketocanazole Singly and in Combination. Journal of Cosmetics, Dermatological Sciences and Applications. 2016;06(03). https://doi.org/10.4236/jcdsa.2016.63014
- Sharquie KE, Najim RA, Al-Dori WS, Al-Hayani RK. Oral zinc sulfate in the treatment of Behcets disease: A double blind cross-over study. The Journal of Dermatology. 2006Jan;33(8). https://doi.org/10.1111/j.1346-8138.2006.00128.x PMid:16923135
- Sharquie KE. Oral Zinc Sulphate in Treatment of Alopecia Areata (Double Blind; Cross-Over Study). Journal of Clinical & Experimental Dermatology Research. 2014;3(3). https://doi.org/10.4172/2155-9554.1000150
How to cite this article
Vancouver
Islam H, Rahman A, Masud J, Shweta DS, Araf Y, Ullah MA, et al. A Generalized Overview of SARS-CoV-2: Where Does the Current Knowledge Stand?. ELECTRON J GEN MED. 2020;17(6):em251. https://doi.org/10.29333/ejgm/8258
APA
Islam, H., Rahman, A., Masud, J., Shweta, D. S., Araf, Y., Ullah, M. A., Sium, S. M. A., & Sarkar, B. (2020). A Generalized Overview of SARS-CoV-2: Where Does the Current Knowledge Stand?. Electronic Journal of General Medicine, 17(6), em251. https://doi.org/10.29333/ejgm/8258
AMA
Islam H, Rahman A, Masud J, et al. A Generalized Overview of SARS-CoV-2: Where Does the Current Knowledge Stand?. ELECTRON J GEN MED. 2020;17(6), em251. https://doi.org/10.29333/ejgm/8258
Chicago
Islam, Hiya, Ahsab Rahman, Jaasia Masud, Dipita Saha Shweta, Yusha Araf, Md. Asad Ullah, Syed Muktadir Al Sium, and Bishajit Sarkar. "A Generalized Overview of SARS-CoV-2: Where Does the Current Knowledge Stand?". Electronic Journal of General Medicine 2020 17 no. 6 (2020): em251. https://doi.org/10.29333/ejgm/8258
Harvard
Islam, H., Rahman, A., Masud, J., Shweta, D. S., Araf, Y., Ullah, M. A., . . . Sarkar, B. (2020). A Generalized Overview of SARS-CoV-2: Where Does the Current Knowledge Stand?. Electronic Journal of General Medicine, 17(6), em251. https://doi.org/10.29333/ejgm/8258
MLA
Islam, Hiya et al. "A Generalized Overview of SARS-CoV-2: Where Does the Current Knowledge Stand?". Electronic Journal of General Medicine, vol. 17, no. 6, 2020, em251. https://doi.org/10.29333/ejgm/8258