TNF-α-308 and INF-γ+874 Gene Polymorphisms in Relation to Susceptibility and Severity of Type 2 Diabetes Mellitus among Egyptian Cases

Afaf Elsaid; Mohamed A. Helaly; El-Sayed Z. Hatata; Osama Fouda; Ahmad Settin

doi:10.29333/ejgm/82455

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Afaf Elsaid ¹, Mohamed A. Helaly ¹ ^* , El-Sayed Z. Hatata ¹, Osama Fouda ¹, Ahmad Settin ¹

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¹ Mansoura University, Mansoura, Egypt^* Corresponding Author

Abstract

To explore the association of TNF-α-308 and IFN- γ +874 genetic polymorphisms with type 2 diabetes mellitus (DM) in Egyptian cases. Participants included 207 cases with type 2 DM, 93 males and 114 females. Their mean age was 57 years. They were compared to a control group of 212 healthy unrelated subjects from the same locality. DNA was amplified using PCR with sequence specific primers for detection of polymorphism related to TNF-α-308 (G/A) and IFN-γ+874 (A/T). The combined homozygote pattern of TNF-α-308 (AA) and IFN-γ+874 (A/A) was significantly higher in diabetic cases versus controls (p=0.023) that was manifest (although non-significant) among complicated, uncontrolled cases and cases with marked insulin resistance. Polymorphism related to TNF-α-308 AA and IFN-γ+874 AA may be considered as a genetic biomarker for type 2 DM in Egyptian subjects with potential impact on family counseling and management.

Keywords

TNF-α-308
IFN- γ +874
type 2 DM
gene polymorphism
Egypt

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Original Article

EUR J GEN MED, 2012, Volume 9, Issue 3, 173-177

https://doi.org/10.29333/ejgm/82455

Publication date: 10 Jul 2012

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Vancouver

Elsaid A, Helaly MA, Hatata ESZ, Fouda O, Settin A. TNF-α-308 and INF-γ+874 Gene Polymorphisms in Relation to Susceptibility and Severity of Type 2 Diabetes Mellitus among Egyptian Cases. EUR J GEN MED. 2012;9(3):173-7. https://doi.org/10.29333/ejgm/82455

APA

Elsaid, A., Helaly, M. A., Hatata, E.-S. Z., Fouda, O., & Settin, A. (2012). TNF-α-308 and INF-γ+874 Gene Polymorphisms in Relation to Susceptibility and Severity of Type 2 Diabetes Mellitus among Egyptian Cases. European Journal of General Medicine, 9(3), 173-177. https://doi.org/10.29333/ejgm/82455

AMA

Elsaid A, Helaly MA, Hatata ESZ, Fouda O, Settin A. TNF-α-308 and INF-γ+874 Gene Polymorphisms in Relation to Susceptibility and Severity of Type 2 Diabetes Mellitus among Egyptian Cases. EUR J GEN MED. 2012;9(3), 173-177. https://doi.org/10.29333/ejgm/82455

Chicago

Elsaid, Afaf, Mohamed A. Helaly, El-Sayed Z. Hatata, Osama Fouda, and Ahmad Settin. "TNF-α-308 and INF-γ+874 Gene Polymorphisms in Relation to Susceptibility and Severity of Type 2 Diabetes Mellitus among Egyptian Cases". European Journal of General Medicine 2012 9 no. 3 (2012): 173-177. https://doi.org/10.29333/ejgm/82455

Harvard

Elsaid, A., Helaly, M. A., Hatata, E.-S. Z., Fouda, O., and Settin, A. (2012). TNF-α-308 and INF-γ+874 Gene Polymorphisms in Relation to Susceptibility and Severity of Type 2 Diabetes Mellitus among Egyptian Cases. European Journal of General Medicine, 9(3), pp. 173-177. https://doi.org/10.29333/ejgm/82455

MLA

Elsaid, Afaf et al. "TNF-α-308 and INF-γ+874 Gene Polymorphisms in Relation to Susceptibility and Severity of Type 2 Diabetes Mellitus among Egyptian Cases". European Journal of General Medicine, vol. 9, no. 3, 2012, pp. 173-177. https://doi.org/10.29333/ejgm/82455

Electronic Journal of General Medicine

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