DOES THE SUBACUTE (4-WEEK) EXPOSURE TO FORMALDEHYDE INHALATION LEAD TO OXIDANT/ANTIOXIDANT IMBALANCE IN RAT LIVER?
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Sadık Söğüt 1 * , Ahmet Songur 2, Oğuz Aslan Özen 2, Hüseyin Özyurt 3, Mustafa Sarsılmaz 4
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1 Mustafa Kemal University, Faculty of Medicine, Department of Biochemistry, Antakya, Turkey2 Afyon Kocatepe University, Faculty of Medicine, Department of Anatomy, Afyon, Turkey3 Gaziosmanpaşa University, Faculty of Medicine, Department of Biochemistry, Tokat, Turkey4 Fırat University, Faculty of Medicine, Department of Anatomy, Elazığ, Turkey* Corresponding Author

Abstract

The aim of this experimental study is to evaluate oxidant/antioxidant status and the end products of lipid peroxidation in the liver of rats exposed to subacut formaldehyde (FA) inhalation (4-weeks). Thirty male Wistar albino rats divided into 3 separate groups randomly (10 for each group). Rats were exposed to atmospheres containing 0, 10 and 20 ppm FA continuously (8 hours per day, 5 days per week). Reduced glutathione (GSH), malondialdehyde (MDA) and nitric oxide (NO) levels, as well as xanthine oxidase (XO), and myeloperoxidase (MPO) activities were measured in rat liver homegenates. The results showed a remarkable reduction of GSH content (10 and 20 ppm FA) and XO activity (20 ppm FA) in liver tissues from rats exposed to for 4- weeks (p<0.005, p<0.0001 and p<0.0001, respectively). MDA and NO levels did not change in both two groups (10 ppm and 20 ppm). These findings suggest that the antioxidant system of liver tissue is moderately impaired by excessive FA exposure. The GSH has an important influence especially on subacute FA liver toxicity. GSH-related cellular defensive mechanisms may be deppressed and susceptibility to oxidative damage may high in rats exposed to FA subacutely.

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Original Article

EUR J GEN MED, 2004, Volume 1, Issue 3, 26-32

https://doi.org/10.29333/ejgm/82199

Publication date: 15 Jul 2004

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