Three-dimensional TCP scaffolds enriched with Erythropoietin for stimulation of vascularization and bone formation
More details
Hide details
I.M. Sechenov First State Medical University, Russia
Lomonosov Moscow State University, Russia
P Hertsen Moscow Oncology Research Institute, Russia
Federal State Budgetary Institution National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Russia
Institute of Metallurgy and Material Science, Russia
Russian Medical Academy of Postgraduate Education, Russia
Online publication date: 2019-04-17
Publication date: 2019-04-17
Electron J Gen Med 2019;16(2):em115
In our work, we conducted studies to evaluate the reconstruction of an artificially created critical radial defect in rats with the use of tri-calcium phosphate scaffold enriched with erythropoietin (ЕРО). A model of the bone defect on the radius forearm in rats of a critical size has been developed. This model allows to conduct the study without the use of osteosynthesis. EPO is a well-known hormone which regulates formation of the red blood cells (2, 3). ЕРО increases the expression of VEGF and promotes angiogenesis (3, 4). Therefore, EPO may have a great potential for use as a growth factor for angiogenesis and play its particular role in the bone tissue regeneration. There are no published studies that describe interactions between EPO and biomaterials for development of the bone tissue. Thus, the purpose of this study was to evaluate the interaction between EPO and tri-calcium phosphate scaffold (TCP) with a well-studied biocompatibility and the given porosity, as well as to determine whether EPO in the enriched TCP will promote the bone regeneration. The X-ray analysis was performed in 10 days, 28 days and 3 months after the surgery. The histological analysis was performed in 28 days and 3 months after the surgery. The results have demonstrated that the complex of TCP scaffold with erythropoietin is a promising growth factor for stimulating the development of the bone tissue, since the TCP scaffold enriched with erythropoietin is very easy to obtain in a non-invasive and simple procedure, as well as the complex promotes interaction with the surrounding tissues and induces the bone regeneration.
Mauffrey C, Barlow BT, Smith W. Management of segmental bone defects. J Am Acad Orthop Surg. 2015;23(3):143-53. https://doi.org/10.5435/JAAOS-... PMid:25716002.
Roddy E, DeBaun MR, Daoud-Gray A, Yang YP, Gardner MJ. Treatment of critical-sized bone defects: clinical and tissue engineering perspectives. Eur J Orthop Surg Traumatol. 2018;28(3):351-62. https://doi.org/10.1007/s00590... PMid:29080923.
Schmitz JP, Hollinger JO. The critical size defect as an experimental model for craniomandibulofacial nonunions. Clin Orthop Relat Res. 1986(205):299-308. https://doi.org/10.1097/000030....
DeBaun MR, Stahl AM, Daoud AI, Pan C-C, Bishop JA, Gardner MJ, Yang YP. Preclinical Induced Membrane Model to Evaluate Synthetic Implants for Healing Critical Bone Defects without Autograft. J Orthop Res. 2019;37(1):60-8. https://doi.org/10.1002/jor.24....
Liao HT, Lee MY, Tsai WW, Wang HC, Lu WC. Osteogenesis of adipose-derived stem cells on polycaprolactone-β-tricalcium phosphate scaffold fabricated via selective laser sintering and surface coating with collagen type I. J Tissue Eng Regen Med 2016;10:E337-53. https://doi.org/10.1002/term.1... PMid:23955935.
Park H, Kim JS, Oh EJ, Kim TJ, Kim HM, Shim JH, Yoon WS, Huh JB, Moon SH, Kang SS, Chung HY. Effects of three-dimensionally printed polycaprolactone/β-tricalcium phosphate scaffold on osteogenic differentiation of adipose tissue- and bone marrow-derived stem cells. Arch Craniofac Surg. 2018;19(3):181–9. https://doi.org/10.7181/acfs.2....
Rose FR, Cyster LA, Grant DM, Scotchford CA, Howdle SM, Shakesheff KM. In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel. Biomaterials 2004;25:5507-14. https://doi.org/10.1016/j.biom... PMid:15142732.
Minoda R, Hayashida M, Masuda M, Yumoto E. Preliminary experience with beta-tricalcium phosphate for use in mastoid cavity obliteration after mastoidectomy. Otol Neurotol 2007;28:1018-21. https://doi.org/10.1097/MAO.0b... PMid:17898672.
Polymer-mineral scaffold augments in vivo equine multipotent stromal cell osteogenesis - Wei Duan, Cong Chen, Masudul Haque, Daniel Hayes, Mandi J. Lopez, Stem Cell Res Ther. 2018;9:60. https://doi.org/10.1186/s13287....
Alfotawei R, Naudi KB, Lappin D, Barbenel J, Di Silvio L, Hunter K, McMahon J, Ayoub A. The use of TriCalcium Phosphate (TCP) and stem cells for the regeneration of osteoperiosteal critical-size mandibular bony defects, an in vitro and preclinical study. Journal of cranio-maxillo-facial surgery. 2014;42(6):863-9. https://doi.org/10.1016/j.jcms....
Cancedda R, Giannoni P, Mastrogiacomo M. A tissue engineering approach to bone repair in large animal models and in clinical practice. Biomaterials 2007;28:4240-50. https://doi.org/10.1016/j.biom... PMid:17644173.
Heliotis M, Lavery KM, Ripamonti U. Transformation of a prefabricated hydroxyapatite/osteogenic protein-1 implant into a vascularised pedicled bone flap in the human chest. Int J Oral Maxillofac Surg 2006;35:265-73. https://doi.org/10.1016/j.ijom... PMid:16257511.
Ayoub AF, Challa R, Abu-Serriah M, McMahan J, Moos K, Creanor S, et al. Use of a composite pedicled muscle flap and rh BMP-7 for mandibular reconstruction. Int J Oral Maxillofac Surg 2007;36:1183-92. https://doi.org/10.1016/j.ijom... PMid:17822878.
Arosarena OA, Malmgren L, Falk A, Bookman LP, Allen MJ, Schoonmaker JO, et al. Defect repair in the rat mandible with bone morphogenic proteins and marrow cells. Arch Facial Plast Surg 2003;5:103-8. https://doi.org/10.1001/archfa... PMid:12533151.
Wilson SM, Goldwasser MS, Clark SG, Monaco E, Bionaz M, Hurley WL, et al. Adipose-derived mesenchymal stem cells enhance healing of mandibular defects in the ramus of swine. J Oral Maxillofac Surg 2012;70:e193-203. https://doi.org/10.1016/j.joms... PMid:22374062.
Henkel KO, Gerber T, Dörfling P, Gundlach KK, Bienengräber V. Repair of bone by applying biomatrices with and without autologous osteoblasts. J Craniofac Surg 2005;33:45-9. https://doi.org/10.1016/j.jcms... PMid:15694149.
He Y, Zhang ZY, Zhu HG, Qiu W, Jiang X, Guo W. Experimental study on reconstruction of segmental mandible defects using tissue engineered bone combined bone marrow stromal cells with three-dimensional tricalcium phosphate. J Craniofac Surg 2007;18:800-5. https://doi.org/10.1097/scs.0b... PMid:17667668.
Ren J, Ren T, Zhao P, Huang Y, Pan K. Repair of mandibular defects using MSCs-seeded biodegradable polyester porous scaffolds. J Biomater Sci Polym Ed 2007;18:505-17. https://doi.org/10.1163/156856... PMid:17550655.
Torroni A. Engineered bone graft and bone flaps for maxillofacial defects: state of art. J Oral Maxillofac Surg 2009;67:1121-7. https://doi.org/10.1016/j.joms... PMid:19375027.
Roldán JC, Detsch R, Schaefer S, Chang E, Kelantan M, Waiss W, et al. Bone formation and degradation of a highly porous biphasic calcium phosphate ceramic in presence of BMP-7, VEGF and mesenchymal stem cells in an ectopic mouse model. J Craniomaxillofac Surg 2010;38:423-30. https://doi.org/10.1016/j.jcms... PMid:20189819.
Maiborodin IV, Matveeva VA, Kolesnikov IS, Drovosekov MN, Toder MS, Shevela AI. Regeneration of red bone marrow in rat lower jaw after transplantation of mesenchymal stem cells into the site of injury. Bull Exp Biol Med 2012;152:528-34. https://doi.org/10.1007/s10517... PMid:22803127.
Vahabi S, Amirizadeh N, Shokrgozar MA, Mofeed R, Mashhadi A, Aghaloo M, et al. A comparison between the efficacy of Bio-Oss, hydroxyapatite tricalcium phosphate and combination of mesenchymal stem cells in inducing bone regeneration. Chang Gung Med J 2012;35:28-37. https://doi.org/10.4103/2319-4....
Guo C, Haider H, Shim WS, Tan RS, Ye L, Jiang S, et al. Myoblast-based cardiac repair: xenomyoblast versus allomyoblast transplantation. J Thorac Cardiovasc Surg. 2007;134:1332–9. https://doi.org/10.1016/j.jtcv... PMid:17976470.