ORIGINAL ARTICLE
STUDY ON PROTEOMICS EXPRESSION PROFILE OF SERA FROM PATIENTS WITH UREMIA
Dai Yong 1  
,  
Wang Jianqing 2
,  
Deng Anguo 2
,  
Liu Jian-jun 3
,  
 
 
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1
Jinan University, Shenzhen People’s Hospital, Second Clinical Medical College, Department of Nephrology, Shenzhen, China
2
Huazhong University of Science and Technology, Union Hospital, Tongji Medical College, Department of Nephrology, Wuhan, China
3
Center for Disease Control and Prevention of Shenzhen, Department of Microorganism Test, Shenzhen, China
CORRESPONDING AUTHOR
Dai Yong   

Clinical Medical Research Center, Shenzhen People's Hospital, Second Clinical Medical College, Jinan University. No 1017, Dongmen north rd, Shenzhen, Guangdong 518020, China Phone: 86 13802201510 Fax: 86 075525626750
Online publish date: 2008-04-15
Publish date: 2008-04-15
 
Eur J Gen Med 2008;5(2):64–73
KEYWORDS
ABSTRACT
Aim: To establish and optimize two-dimensional electrophoresis (2DE) and relevant techniques for the study of serum proteome of the patients with uremia, and compare serum protein 2DE pattern between the uremia patients and the normal. Methods: Using immobiline pH gradients isoelectricfocusing (IEF) as the 1st dimension and vertical SDS-PAGE as the 2nd dimension, we performed the comparison and conditional optimization of the factors. Immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis,silver staining, ImageMaster 2D 5.0 analysis software, matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-TOF-MS) and IPI human database searching, were used to separate and indentify the proteome of the sera from the patients with uremia. Results: Satisfactory 2DE patterns of the serum proteins were obtained. 26 protein spots were remarkably hanged in uremia patients, and 20 protein spots were identified by MALDI-TOF-TOF-MS.. Conclusion: Good reproducibility could be obtained by applying immobilized pH gradient 2DE to separate and identify the proteome in sera, which contributes to the further study on uremia toxins pertaining to protein.
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