ORIGINAL ARTICLE
Prediction of biomarker miRNAs signature in colorectal cancer metastasis to liver cancer
Z. Salehi 1
,  
P. Haddad 2
,  
Omid Tavallaei 1, 2  
 
 
More details
Hide details
1
Department of pharmacognosy and pharmaceutical biotechnology, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
2
Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
Online publish date: 2018-07-14
 
KEYWORDS:
ABSTRACT:
Introduction:
microRNAs (miRNAs) are frequently dysregulated in colorectal cancer (CRC) primary tumors vs. metastasic to the liver.

Objective:
Our aim was to prediction of biomarker miRNAs signature of CRC Metastasis to liver cancer.

Material and Methods:
mRNA and miRNA expression profiles of CRC primary tumors to metastases formed in the liver were downloaded from NCBI Gene Expression Omnibus (GEO) database. miRNAs targets were predicted using Targetscan algorithm. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) enrichment analysis were performed using DAVID online tool, since mRNA expression profile of liver and colon cancer primary tumors vs. metastasis to the liver were used in background of these analysis.

Results:
43 and 58 down- and up-regulated miRNAs were obtained from GSE98406 (p-value < 0.05). mRNA expression profile include of 1,048 mRNAs differentially expressed in metastatic and non-metastatic CRC to liver from GSE40367 (p-value < 0.05). The some of the down-regulated miRNAs were significantly enriched in migration signaling and cancer stem cell signaling. Moreover, the some of the up-regulated miRNAs were significantly enriched in negative regulation of cell migration.

Conclusions:
Some of the miRNAs are many number of target genes that some of these are oncogenes and tumor suppressor genes. It is concluded that differentially expressed miRNAs in metastatic vs. non-metastatic CRC to the liver take part in cell migration and cancer stem cell signaling pathways.

 
REFERENCES (15):
1. Calin GA, Croce CM. MicroRNA signatures in human cancers. Nature reviews cancer, 2006;6(11):857-866. https://doi.org/10.1038/nrc199....
2. Kim VN. MicroRNA biogenesis: coordinated cropping and dicing. Nature reviews Molecular cell biology, 2005;6(5):376-385. https://doi.org/10.1038/nrm164....
3. Banno K., et al. MicroRNA in cervical cancer: OncomiRs and tumor suppressor miRs in diagnosis and treatment. The Scientific World Journal. 2014. https://doi.org/10.1155/2014/1....
4. Ricci-Vitiani L., et al. Identification and expansion of human colon-cancer-initiating cells. Nature, 2007;445(7123):111-115. https://doi.org/10.1038/nature....
5. Chen X., et al. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell research. 2008;18(10):997-1006. https://doi.org/10.1038/cr.200....
6. Luo X., et al. MicroRNA signatures: novel biomarker for colorectal cancer? Cancer Epidemiology and Prevention Biomarkers, 2011. 20(7): p. 1272-1286. https://doi.org/10.1158/1055-9....
7. Fidler IJ. The pathogenesis of cancer metastasis: the ‘seed and soil’ hypothesis revisited. Nature Reviews Cancer. 2003;3(6):453-458. https://doi.org/10.1038/nrc109....
8. Hurst DR, Edmonds MD, Welch DR. Metastamir: the field of metastasis-regulatory microRNA is spreading. Cancer research. 2009;69(19):7495-7498. https://doi.org/10.1158/0008-5....
9. Arakawa K., et al. KEGG-based pathway visualization tool for complex omics data. In silico biology. 2005;5(4):419-423.
10. Hulsegge I, Kommadath A, Smits MA. Globaltest and GOEAST: two different approaches for Gene Ontology analysis. In BMC proceedings. 2009. BioMed Central. https://doi.org/10.1186/1753-6....
11. Huang DW, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nature protocols. 2009;4(1):44-57. https://doi.org/10.1038/nprot.....
12. Sheng W-Z, et al. MicroRNA-21 promotes phosphatase gene and protein kinase B/phosphatidylinositol 3-kinase expression in colorectal cancer. World journal of gastroenterology. 2016;22(24):5532. https://doi.org/10.3748/wjg.v2....
13. Longqiu Y, et al. A miRNAs panel promotes the proliferation and invasion of colorectal cancer cells by targeting GABBR1. Cancer medicine. 2016;5(8):2022-2031. https://doi.org/10.1002/cam4.7....
14. Bakirtzi K, et al. Neurotensin signaling activates microRNAs-21 and-155 and Akt, promotes tumor growth in mice, and is increased in human colon tumors. Gastroenterology. 2011;141(5):1749-1761. https://doi.org/10.1053/j.gast....
15. Qin J, Luo M. MicroRNA‐221 promotes colorectal cancer cell invasion and metastasis by targeting RECK. FEBS letters. 2014;588(1):99-104. https://doi.org/10.1016/j.febs....
eISSN:2516-3507